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Title: Fetal gene transfer with lentiviral vectors: long-term in vivo follow-up evaluation in a rat model
Authors: Toelen, Jaan ×
Deroose, Christophe
Gijsbers, Rik
Reumers, Veerle
Sbragia, Lourenço N
Vets, Sofie
Chitneni, Satish Kumar
Bormans, Guy
Mortelmans, Luc
Deprest, Jan
Debyser, Zeger #
Issue Date: 22-Apr-2007
Publisher: Mosby, Inc.
Series Title: American Journal of Obstetrics and Gynecology vol:196 issue:4 pages:352.e1-6
Abstract: OBJECTIVE: The purpose of this study was to evaluate the long-term expression of a transgene and subsequent immune response after the injection of lentiviral vectors in a fetal rats. STUDY DESIGN: Fetal rats were injected in the liver, peritoneal cavity, or lung at E19 (term, E21) with a lentiviral vector expressing enhanced green fluorescent protein and luciferase. Controls received saline solution. After birth, full body bioluminescence was done at weeks 1, 4, 10, and 30 of life; seroconversion for the transgene was assessed. RESULTS: All surviving fetuses that had been injected in the liver (8/9 fetuses), peritoneum (3/3 fetuses), or lung (9/10 fetuses) showed a signal on bioluminescence imaging scan up to 30 weeks. None of the survivors displayed seroconversion against the transgene. CONCLUSION: In the rat model, the administration of lentiviral vectors into the fetal lung and liver resulted in long-term transgene expression without detectable humoral immune response.
URI: 
ISSN: 0002-9378
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Basic Research in Gynaecology Section (-)
Clinical Residents Medicine
Research Group for Neurobiology and Gene Therapy
Radiopharmacy
Nuclear Medicine & Molecular Imaging
Molecular Virology and Gene Therapy
Foetal Medicine Section (-)
× corresponding author
# (joint) last author

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