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Title: The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcriptional activation
Authors: Meersseman, Geert ×
Verschueren, K
Nelles, Lucien
Blumenstock, C
Kraft, Henricus
Wuytens, Gunther
Remacle, Jacques
Kozak, C A
Tylzanowski, Przemyslaw
Niehrs, C
Huylebroeck, Danny #
Issue Date: May-1997
Series Title: Mechanisms of development. vol:61 issue:1-2 pages:127-40
Abstract: We report the characterization of two vertebrate homologs of Drosophila mothers against dpp (Mad) isolated from the mouse and the Xenopus embryo, named MusMLP (mad-like protein) and XenMLP, respectively, together with a summary of their expression patterns in the embryo. Overexpression of XenMLP causes ventralization of Xenopus embryos and we demonstrate that the C-terminal domain is necessary and sufficient to confer this biological effect. This domain also has the potential for transcriptional activation, as shown in one-hybrid assays in mammalian cells. We further demonstrate that MLPs are multidomain proteins by showing a cis-negative effect of the N-terminal domain on the transactivation by the C-terminal domain and that the proline-rich, middle domain maximizes the activity of the C-terminal domain. We also mapped the MusMLP gene to a region on mouse chromosome 13 that corresponds to a region on human chromosome 5q that contains cancer-related genes.
ISSN: 0925-4773
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Biology (Celgen) (-)
Rheumatology Section (-)
× corresponding author
# (joint) last author

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