Author:

Keywords:

Alkaline Phosphatase, Animals, Bile Ducts, Cytosine Nucleotides, Liver, Microscopy, Electron, Rats

Abstract:

Since in histochemical reactions the liver alkaline phosphatase discloses only low activity with respect to beta-glycerophosphate, the authors have replaced the latter by cytidine monophosphate. With this substrate they obtained much better results: greater sensibility permitting to reduce the incubation time, and much more precise localization of the enzyme activity, at the ultrastructural level. Details of the method are given with a discussion of its specifity. The method was applied on liver tissue of normal albino rats and animals of the same strain with ligated common bile duct. © 1967 Springer-Verlag.