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Title: Interaction of the protein phosphatase 2A with the regulatory domain of the cystic fibrosis transmembrane conductance regulator channel
Authors: Vastiau, Annick ×
Cao, Lishuang
Jaspers, Martine
Owsianik, Grzegorz
Janssens, Veerle
Cuppens, Harry
Goris, Jozef
Nilius, Bernd
Cassiman, Jean-Jacques #
Issue Date: Jun-2005
Publisher: Elsevier on behalf of the Federation of European Biochemical Societies
Series Title: FEBS Letters vol:579 issue:16 pages:3392-3396
Abstract: A direct interaction of the regulatory domain (R domain) of the cystic fibrosis transmembrane conductance regulator protein (CFTR) with PR65, a regulatory subunit of the protein phosphatase 2A (PP2A), was shown in yeast two hybrid, pull-down and co-immunoprecipitation experiments. The R domain could be dephosphorylated by PP2A in vitro. Overexpression of the interacting domain of PR65 in Caco-2 cells, as well as treatment with okadaic acid, showed a prolonged deactivation of the chloride channel. Taken together our results show a direct and functional interaction between CFTR and PP2A.
ISSN: 0014-5793
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Human Mutations and Polymorphisms Section (-)
Biochemistry Section (Medicine) (-)
Laboratory of Ion Channel Research
Laboratory of Protein Phosphorylation and Proteomics
Department of Cellular and Molecular Medicine - miscellaneous
Forensic Biomedical Sciences
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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