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Title: Generation of a floxed allele of Smad5 for cre-mediated conditional knockout in the mouse
Authors: Umans, Lieve
Vermeire, Liesbeth
Francis, Annick
Chang, Hua
Huylebroeck, Danny ×
Zwijsen, An #
Issue Date: Sep-2003
Publisher: John Wiley & Sons, Inc.
Series Title: Genesis: The Journal of Genetics and Development vol:37 issue:1 pages:5-11
Abstract: Smad5 is a member of the Smad family of intracellular mediators of BMP signals and in endothelial cells of TGF-beta signals. We and others previously showed that loss of Smad5 in the mouse results in embryonic lethality (between E9.5-E11.5) due to multiple embryonic and extraembryonic defects. To circumvent the early embryonic lethality and to allow tissue- and time-specific Smad5 inactivation, we created a conditional Smad5 allele in the mouse. Floxed Smad5 (Smad5(flE2,Neo/flE2,Neo)) mice were generated in which both exon2 and the Neo-cassette were flanked by loxP sites. Here we demonstrate that embryos with ubiquitous Cre-mediated deletion of Smad5 (Smad5(flDeltaE2/flDeltaE2)) phenocopy the conventional Smad5 knockout mice. Smad5(flE2/flE2) mice are now available and will be a valuable tool to analyze the role of Smad5 beyond its crucial early embryonic function throughout development and postnatal life.
URI: 
ISSN: 1526-954X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Biology (Celgen) (-)
Embryo and Stemcells (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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