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Title: PIP(2)-PDZ domain binding controls the association of syntenin with the plasma membrane
Authors: Zimmermann, Pascale ×
Meerschaert, Kris
Reekmans, Gunter
Leenaerts, Iris
Small, J Victor
Vandekerckhove, Joël
David, Guido
Gettemans, Jan #
Issue Date: Jun-2002
Publisher: Cell Press
Series Title: Molecular Cell vol:9 issue:6 pages:1215-1225
Abstract: PDZ proteins organize multiprotein signaling complexes. According to current views, PDZ domains engage in protein-protein interactions. Here we show that the PDZ domains of several proteins bind phosphatidylinositol 4,5-bisphosphate (PIP(2)). High-affinity binding of syntenin to PIP(2)-containing lipid layers requires both PDZ domains of this protein. Competition and mutagenesis experiments reveal that the protein and the PIP(2) binding sites in the PDZ domains overlap. Overlay assays indicate that the two PDZ domains of syntenin cooperate in binding to cognate peptides and PIP(2). Experiments on living cells demonstrate PIP(2)-dependent and peptide-dependent modes of plasma membrane association of the PDZ domains of syntenin. These observations suggest that local changes in phosphoinositide concentration control the association of PDZ proteins with their target receptors at the plasma membrane.
URI: 
ISSN: 1097-2765
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Genetics Section (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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