This item still needs to be validated !
Title: The preservation and regeneration of cilia on human nasal epithelial cells cultured in vitro
Authors: Jorissen, Mark
Van der Schueren, Bernadette
Van den Berghe, Herman
Cassiman, Jean-Jacques #
Issue Date: Jan-1990
Series Title: Archives of oto-rhino-laryngology. vol:246 issue:5 pages:308-14
Abstract: Dissociated human nasal epithelial cells from nasal polyps were cultured in Ham's F12-DME 1/1 supplemented with NU-serum 10%, choleratoxin (10 ng/ml), retinoic acid (10(-7) M) and antibiotics. In monolayer cultures, the epithelial cells grew to confluency on collagen gels, became squamous, and lost their cilia within 2-6 weeks. In suspension cultures, epithelial cell sheaths formed stable vesicles and aggregates. These maintained a respiratory-type morphology and normal ciliary activity for over 6 months. When deciliated, squamous cells from monolayer cultures were brought in suspension, a respiratory-type morphology with cilia reappeared. This in vitro ciliogenesis resulted in normal and coordinated ciliary activity observed for more than 5 months.
ISSN: 0302-9530
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Human Mutations and Polymorphisms Section (-)
Research Group Experimental Oto-rhino-laryngology
Clinical Genetics Section (-)
Forensic Biomedical Sciences
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science