Title: Oligosaccharides of recombinant mouse gelatinase B variants
Authors: Van den Steen, Philippe
Rudd, PM
Proost, Paul
Martens, Erik
Paemen, L
Kuster, B
Van Damme, Jozef
Dwek, RA
Opdenakker, Ghislain # ×
Issue Date: Nov-1998
Publisher: Elsevier
Series Title: Biochimica et Biophysica Acta. General Subjects vol:1425 issue:3 pages:587-98
Abstract: Gelatinase B (matrix metalloproteinase-9, MMP-9) contains three N-glycosylation sites and a Ser/Thr/Pro-rich type V collagen domain with repetitive attachment sites for O-linked sugars. Recombinant mouse gelatinase B was expressed in the yeast Pichia pastoris and the N-linked oligosaccharides of the truncated glycoprotein variants were analysed by in gel enzymatic release followed by mass spectrometry and normal phase HPLC. This technology, despite of the limiting amount of material, allowed the analysis of the formula of N- and O-linked sugars of the different glycoprotein variants. The 112/99- and 88-kDa gelatinase B forms each contained an oligomannose series (Man(8)GlcNAc(2) to Man(15)GlcNAc(2)). Analysis of the hydrazine-released sugars showed that the O-linked oligosaccharides contained alpha 1-2, alpha 1-3 or alpha 1-6 linked mannoses. These results were confirmed by lectin blot analysis of intact and glycosidase-treated enzyme variants. (C) 1998 Elsevier Science B.V. All rights reserved.
ISSN: 0304-4165
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Immunology (Rega Institute)
Laboratory of Immunobiology (Rega Institute)
Biochemistry Section (Medicine) (-)
Leuven Language Institute (ILT)
Academic Center for General Practice
× corresponding author
# (joint) last author

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