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Title: Successful use of the same slide for consecutive fluorescence in situ hybridization experiments
Authors: Dierlamm, J ×
Wlodarska, Iwona
Michaux, Lucienne
La Starza, R
Zeller, W
Mecucci, Christina
Van den Berghe, Herman #
Issue Date: Jan-1997
Series Title: Genes, chromosomes & cancer. vol:16 issue:4 pages:261-4
Abstract: The feasibility of using the same slide repeatedly for fluorescence in situ hybridization (FISH) experiments was systematically evaluated by applying standard procedures and various combinations of direct- and indirect-labeled probes to slides from patients with hematologic malignancies. Specific and distinct hybridization signals along with weak background signals and chromosome morphology of good to moderate quality could be obtained in up to three experiments performed consecutively on the same slide. Signals related to biotin- or digoxigenin-labeled probes applied in previous hybridizations were still visible with variable intensity, but interpretation problems that may result from this signal noise can be avoided by using adequate probes, detection systems and fluorochromes, and sequence of experiments.
ISSN: 1045-2257
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical Genetics Section (-)
Laboratory for Genetics of Malignant Disorders
Clinical Genetics
× corresponding author
# (joint) last author

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