Characterisation of antibiotic moenomycin A interaction with phospholipid model membranes
Volke, F × Waschipky, R Pampel, A Donnerstag, A Lantzsch, G Pfeiffer, Helge Richter, W Klose, G Welzel, P #
Elsevier sci ireland ltd
Chemistry and physics of lipids vol:85 issue:2 pages:115-123
Using a combination of physico-chemical techniques (MAS NMR, DSC, freeze-fracture electron microscopy, molecular modelling) the antibiotic moenomycin A was found to be anchored by its hydrophobic chain into multilamellar POPC membranes. The lamellar phase structure of the modified membrane is retained, while moenomycin A in water at different concentrations does not form any other but isotropic phase structures. The mobility of POPC molecule segments is reduced with increasing moenomycin A concentrations. Freeze-fracture electron microscopy images show ripple like structures for low moenomycin A concentrations, which are rare for high concentrations. A sugar-group network of the antibiotic seems to cover the whole membrane surface for molar ratios moenomycin A/POPC of 1:2, which is supported by C-13-MAS (Magic Angle Spinning) NMR, P-31-NMR, and molecular modelling. (C) 1997 Elsevier Science Ireland Ltd.