This item still needs to be validated !
Title: Molecular cloning of the mouse gene coding for alpha 2-macroglobulin and targeting of the gene in embryonic stem cells
Authors: Umans, Lieve
Serneels, Lutgarde
Hilliker, Carl
Stas, L
Overbergh, Lutgart
De Strooper, Bart
Van Leuven, Freddy ×
Van den Berghe, Herman #
Issue Date: Jan-1995
Series Title: Genomics. vol:22 issue:3 pages:519-29
Abstract: We have cloned the mouse gene coding for alpha 2-macroglobulin in overlapping lambda clones and have analyzed its structure. The gene contains 36 exons, coding for the 4.8-kb cDNA that we cloned previously. Including putative control elements in the 5' flanking region, the gene covers about 45 kb. A region of 3.8 kb, stretching from 835 bases upstream of the cDNA start site to exon 4, including all intervening sequences, was sequenced completely. The analysis demonstrated that the putative promoter region of the mouse A2M gene differed considerably from the known promoter sequences of the human A2M gene and of the rat acute-phase A2M gene. Comparison of the exon-intron structure of all known genes of the A2M family confirmed that the rat acute phase A2M gene is more closely related to the human gene than to the mouse A2M gene. To generate mice with the A2M gene inactivated, an insertion type of construct containing 7.5 kb of genomic DNA of the mouse strain 129/J, encompassing exons 16 to 19, was synthesized. A hygromycin marker gene was embedded in intron 17. After electroporation, 198 hygromycin-resistant ES cell lines were isolated and analyzed by Southern blotting. Five ES cell lines were obtained with one allele of the mouse A2M gene targeted by this insertion construct, demonstrating that the position and the characteristics of the vector served the intended goal.
ISSN: 0888-7543
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Genetics Section (-)
Associated Laboratories - miscellaneous (-)
Clinical and Experimental Endocrinology
Clinical Genetics Section (-)
Embryo and Stemcells (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science