Title: N-terminal 10- and 12-kDa POMC fragments stimulate differentiation of lactotrophs
Authors: Van Bael, Annemie ×
Vande Vijver, Veerle
Devreese, B
Denef, Carl #
Issue Date: 1996
Publisher: Pergamon-elsevier science ltd
Series Title: Peptides vol:17 issue:7 pages:1219-1228
Abstract: Medium conditioned by a highly enriched population of gonadotrophs, cultured as reaggregates in the presence of 0.01 nM GnRH, was concentrated, separated on a reversed-phase HPLC column, and tested for activity on lactotroph development in pituitary reaggregate cell cultures of 14-day-old rats. The number of cells expressing prolactin (PRL) mRNA was estimated by image analysis after in situ hybridization of paraffin-embedded sections. The number of these cells entering the mitotic cycle was estimated by autoradiography of [H-3]thymidine ([H-3]T) incorporation. One HPLC column fraction expanded the section area occupied by PRL. mRNA cells without displaying an effect on [H-3]T labeling of these cells, indicating that this fraction induces differentiation in the lactotroph Lineage. The latter fraction was further purified on a second reversed-phase HPLC column, a gel filtration column, and a final reversed-phase HPLC column. From the last column, four substances were isolated that all selectively induced differentiation of lactotrophs. Each of them had an N-terminal amino acid sequence identical to the N-terminal domain of rat proopiomelanocortin (POMC). As determined by mass spectrometric analysis, the M(r)s were 10,091, 10,289, 12,238, and 12,247 Da, respectively. The C-terminal extension of these compounds is possibly up to Gln(74) for the former two compounds and up to Gly(95) for the latter two. Authentic purified human POMC(1-76) mimicked the effects of the purified 10- and 12-kDa rat POMC fragments. The present data suggest that certain isoforms of rat POMC(1-74) and human POMC(1-76) can stimulate lactotroph growth through a differentiation-inducing action on progenitor cells. Copyright (C) 1996 Elsevier Science Inc.
ISSN: 0196-9781
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmacology Section (-)
× corresponding author
# (joint) last author

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