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Title: Cell-surface heparan-sulfate proteoglycans from human vascular endothelial-cells - core protein characterization and antithrombin-iii binding-properties
Authors: Mertens, Griet
Cassiman, Jean-Jacques
Vandenberghe, Herman
Vermylen, Jozef
David, Guido # ×
Issue Date: Oct-1992
Publisher: Amer soc biochemistry molecular biology inc
Series Title: Journal of Biological Chemistry vol:267 issue:28 pages:20435-20443
Abstract: Human aortic endothelial cells (HAEC) and human umbilical vein endothelial cells (HUVEC) were labeled with (SO42-)-S-35 for 48 h. The membrane-associated proteoglycans were solubilized from these monolayers with detergent and purified by ion-exchange chromatography on Mono Q, incorporation in liposomes, and gel filtration. The liposome-intercalated proteoglycans were I-125-iodinated and treated with heparitinase before SDS-polyacrylamide gel electrophoresis. Radiolabeled proteins with apparent molecular masses of 130, 60, 46, 35, and 30 kDa (HAEC) and 180, 130, 62, 43, and 35 kDa (HUVEC) were detected by autoradiography. Further characterization by affinity chromatography on immobilized monoclonal antibodies and by Northern blot analysis provided evidence for the expression of syndecan, glypican, and fibroglycan in human endothelial cells. Most of the heparan sulfate which accumulated in the subendothelial matrix was implanted on a 400-kDa core protein. This protein was immunologically related to perlecan and bound to fibronectin. Binding studies on immobilized antithrombin III suggested that all membrane-associated heparan sulfate proteoglycan forms had the capacity to bind to antithrombin III but that high affinity binding was more typical for glypican. Most of the proteoglycans isolated from the extracellular matrix also bound only with low affinity to antithrombin III. These results imply that glypican may specifically contribute to the antithrombotic properties of the vascular wall.
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
Molecular and Vascular Biology
Human Mutations and Polymorphisms Section (-)
Molecular Genetics Section (-)
Forensic Biomedical Sciences
Faculty of Medicine - miscellaneous
× corresponding author
# (joint) last author

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