Author:

Keywords:

Base Sequence, DNA, Neoplasm, Female, Fusion Proteins, bcr-abl, Humans, Karyotyping, Middle Aged, Molecular Sequence Data, Multiple Myeloma, Oligonucleotide Probes, Philadelphia Chromosome, Polymerase Chain Reaction, RNA, Messenger, Transcription, Genetic, Science & Technology, Life Sciences & Biomedicine, Oncology, Hematology, ACUTE LYMPHOBLASTIC-LEUKEMIA, CHRONIC MYELOGENOUS LEUKEMIA, ABL ONCOGENE, CHROMOSOME, BCR, BREAKPOINTS, GENES, FORMS, CML, 1103 Clinical Sciences, 1112 Oncology and Carcinogenesis, Immunology, 3201 Cardiovascular medicine and haematology, 3202 Clinical sciences, 3211 Oncology and carcinogenesis

Abstract:

Philadelphia positive multiple myeloma is a very rare event and, so far, no molecular data about the involvement of the BCR and C-ABL genes are available. We report here the case of a 64-year-old woman presenting with a typical multiple myeloma and a complex Philadelphia (Ph) chromosome that we investigated at a molecular level using conventional DNA techniques and the polymerase chain reaction (PCR). No rearrangement was observed within the major breakpoint cluster region (M-BCR) although she was found to have a P190 BCR/ABL hybrid transcript using PCR. As far as we know, this is the first description of a P190-type mRNA in a patient with a chronic lymphoid disorder. Since P190 is almost always associated in man with acute forms of hematological malignancies, this suggests that other factors may play a role in determining the phenotype of the disease.