Title: Molecular cloning of the lectin and a lectin-related protein from common Solomon's seal (Polygonatum multiflorum)
Authors: Van Damme, E J ×
Barre, A
Rougé, P
Van Leuven, Freddy
Balzarini, Jan
Peumans, W J #
Issue Date: Oct-1996
Series Title: Plant molecular biology. vol:31 issue:3 pages:657-72
Abstract: The most prominent protein of Polygonatum multiflorum (common Solomon's seal) rhizomes has been identified as a mannose-binding lectin. Analysis of the purified lectin demonstrated that it is a tetramer of four identical subunits of 14 kDa. Molecular cloning further revealed that the lectin from this typical Liliaceae species belongs to the superfamily of monocot mannose-binding proteins. Screening of cDNA libraries constructed with RNA isolated from buds, leaves and flowers of P. multiflorum also yielded cDNA clones encoding a protein, which contains two tandemly arranged domains with an obvious sequence homology to the mannose-binding lectins. Molecular modelling of the Polygonatum lectin and lectin-related protein indicated that the three-dimensional structure of both proteins strongly resembles that of the snowdrop lectin. In addition, this approach suggested that the presumed carbohydrate-binding sites of the lectin can accommodate a mannose residue whereas most of the carbohydrate-binding sites of the lectin-related protein cannot.
ISSN: 0167-4412
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Associated Laboratories - miscellaneous (-)
Laboratory of Virology and Chemotherapy (Rega Institute)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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