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Title: Syntenin, a PDZ protein that binds syndecan cytoplasmic domains
Authors: Grootjans, Jan-Johannes *
Zimmermann, Pascale *
Reekmans, Gunter
Smets, Anne
Degeest, Gisèle
Dürr, Joachim
David, Guido # ×
Issue Date: Dec-1997
Series Title: Proceedings of the National Academy of Sciences of the United States of America vol:94 issue:25 pages:13683-8
Abstract: The syndecans are transmembrane proteoglycans that place structurally heterogeneous heparan sulfate chains at the cell surface and a highly conserved polypeptide in the cytoplasm. Their versatile heparan sulfate moieties support various processes of molecular recognition, signaling, and trafficking. Here we report the identification of a protein that binds to the cytoplasmic domains of the syndecans in yeast two-hybrid screens, surface plasmon resonance experiments, and ligand-overlay assays. This protein, syntenin, contains a tandem repeat of PDZ domains that reacts with the FYA C-terminal amino acid sequence of the syndecans. Recombinant enhanced green fluorescent protein (eGFP)-syntenin fusion proteins decorate the plasmamembrane and intracellular vesicles, where they colocalize and cosegregate with syndecans. Cells that overexpress eGFP-syntenin show numerous cell surface extensions, suggesting effects of syntenin on cytoskeleton-membrane organization. We propose that syntenin may function as an adaptor that couples syndecans to cytoskeletal proteins or cytosolic downstream signal-effectors.
ISSN: 0027-8424
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Genetics Section (-)
Department of Human Genetics - miscellaneous
* (joint) first author
× corresponding author
# (joint) last author

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