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Title: Characterization of a helicase-like transcription factor involved in the expression of the human plasminogen activator inhibitor-1 gene
Authors: Ding, H
Descheemaeker, K
Marynen, Peter
Nelles, Lucien
Carvalho, T
Carmo-Fonseca, M
Collen, Desire
Belayew, Alexandra #
Issue Date: Jun-1996
Series Title: DNA and cell biology. vol:15 issue:6 pages:429-42
Abstract: A 5.4-kb cDNA encoding the protein that binds to the B Box of the plasminogen activator inhibitor-1 (PAI-1) gene was isolated and sequenced. The protein, named helicase-like transcription factor (HLTF), contains a DNA-binding domain, a RING finger domain, and seven helicase domains and is homologous to SWI/SNF proteins. Two HLTF mRNAs of 5.5 and 4.5 kb were detected in most human tissues, a single gene was located on chromosome 3q24-25, and the protein was located in the nucleoplasm. Two HLTF proteins differing in translation start site (Met-1 or Met-123) were obtained by in vitro translation in reticulocyte lysate or by immunoprecipitation from HeLa cell nuclear extracts. In vitro transcription from the PAI-1 promoter in HeLa cell extracts was inhibited by HLTF antibodies and by the HLTF DNA binding domain. Over-expression of HLTF or HLTFMet123 produced a three-fold induction of PAI-1-LUC transient expression in HeLa cells. Mutation of the PAI-1 B Box led to an eight-fold reduction of basal PAI-1-LUC expression in these cell lines, but did not affect the four- to six-fold induction by phorbol esters.
ISSN: 1044-5498
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Genetics Section (-)
Molecular and Vascular Biology
Department of Human Genetics - miscellaneous
# (joint) last author

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