Title: STR analysis of artificially degraded DNA-results of a collaborative European exercise
Authors: Schneider, Peter M ×
Bender, Klaus
Mayr, Wolfgang R
Parson, Walther
Hoste, Bernadette
Decorte, Ronny
Cordonnier, Jan
Vanek, Daniel
Morling, Niels
Karjalainen, Matti
Marie-Paule Carlotti, C
Sabatier, Myriam
Hohoff, Carsten
Schmitter, Hermann
Pflug, Werner
Wenzel, Rainer
Patzelt, Dieter
Lessig, Rüdiger
Dobrowolski, Peter
O'Donnell, Geraldine
Garafano, Luciano
Dobosz, Marina
De Knijff, Peter
Mevag, Bente
Pawlowski, Ryszard
Gusmão, Leonor
Conceicao Vide, Maria
Alonso Alonso, Antonio
García Fernández, Oscar
Sanz Nicolás, Pilar
Kihlgreen, Ann
Bär, Walter
Meier, Verena
Teyssier, Anne
Coquoz, Raphael
Brandt, Conxita
Germann, Ursula
Gill, Peter
Hallett, Justine
Greenhalgh, Matthew #
Issue Date: Jan-2004
Publisher: Elsevier
Series Title: Forensic science international vol:139 issue:2-3 pages:123-34
Abstract: Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. Experiences with degraded DNA from casework samples show that every sample may exhibit different properties in this respect, and that it is difficult to systematically assess the performance of routinely used typing systems for the analysis of degraded DNA samples. Using a batch of artificially degraded DNA with an average fragment size of approx. 200 bp a collaborative exercise was carried out among 38 forensic laboratories from 17 European countries. The results were assessed according to correct allele detection, peak height and balance as well as the occurrence of artefacts. A number of common problems were identified based on these results such as strong peak imbalance in heterozygous genotypes for the larger short tandem repeat (STR) fragments after increased PCR cycle numbers, artefact signals and allelic drop-out. Based on the observations, strategies are discussed to overcome these problems. The strategies include careful balancing of the amount of template DNA and the PCR cycle numbers, the reaction volume and the amount of Taq polymerase. Furthermore, a careful evaluation of the results of the fragment analysis and of automated allele calling is necessary to identify the correct alleles and avoid artefacts.
ISSN: 0379-0738
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Human Mutations and Polymorphisms Section (-)
Forensic Biomedical Sciences
× corresponding author
# (joint) last author

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