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Title: Non-selective cation and dysfunctional chloride channels in the apical membrane of nasal epithelial cells cultured from cystic fibrosis patients
Authors: Jorissen, Mark ×
Vereecke, Johan
Carmeliet, Edward
Van den Berghe, Herman
Cassiman, Jean-Jacques #
Issue Date: Feb-1991
Series Title: Biochimica et Biophysica Acta vol:1096 issue:1 pages:52-9
Abstract: Chloride channels and non-selective cation channels in the apical membranes of cultured nasal epithelial cells from three cystic fibrosis patients were investigated with the patch-clamp technique. Outwardly rectifying chloride channels were found in 31% of the inside-out patches, but activity of this channel was never observed in cell-attached patches, even after stimulation with adrenaline. In 30% of the patches with chloride channels, activation occurred immediately after excision. Most of the channels, however, activated only after a membrane depolarization of +40 to +120 mV. Once activated, the chloride channels were indistinguishable from those in nasal epithelial cells of control patients. Amiloride-insensitive, calcium- and voltage-dependent, non-selective cation channels were present in 11% of the cell-attached and 43% of the cell-free patches and could not be distinguished from those in controls. The cystic fibrosis chloride channel defect is conserved in cultured nasal epithelial cells, while a non-selective cation channel is apparently not affected.
ISSN: 0006-3002
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Human Mutations and Polymorphisms Section (-)
Research Group Experimental Oto-rhino-laryngology
Clinical Genetics Section (-)
Physiology Section (-)
Laboratory of Molecular and Cellular Signaling
Department of Cellular and Molecular Medicine - miscellaneous
Forensic Biomedical Sciences
× corresponding author
# (joint) last author

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