Title: Electrostimulation enhances FAT/CD36-mediated long-chain fatty acid uptake by isolated rat cardiac myocytes
Authors: Luiken, JJFP ×
Willems, Jean
Van der Vusse, GJ
Glatz, JFC #
Issue Date: Oct-2001
Publisher: Amer physiological soc
Series Title: American journal of physiology-endocrinology and metabolism vol:281 issue:4 pages:E704-E712
Abstract: We investigated palmitate uptake and utilization by contracting cardiac myocytes in suspension to explore the link between long-chain fatty acid (FA) uptake and cellular metabolism, in particular the role of fatty acid translocase (FAT)/CD36-mediated transsarcolemmal FA transport. For this, an experimental setup was developed to electrically stimulate cardiomyocytes in multiple parallel incubations. Electrostimulation at voltages greater than or equal to 170 V resulted in, cellular contraction with no detrimental effect on cellular integrity. At 200 V and 4 Hz, palmitate uptake (measured after 3-min incubation) was enhanced 1.5-fold. In both quiescent and contracting myocytes, after their uptake, palmitate was largely and rapidly esterified, mainly into triacylglycerols. Palmitate oxidation (measured after 30 min) contributed to 22% of palmitate taken up by quiescent cardiomyocytes and, after stimulation at 4 Hz, was increased 2.8-fold to contribute to 39% of palmitate utilization. The electrostimulation-mediated increase in palmitate uptake was blocked in the presence of either verapamil, a contraction inhibitor, or sulfo-N-succinimidyl-FA esters, specific inhibitors of FAT/CD36. These data indicate that, in contracting cardiac myocytes, palmitate uptake is increased due to, increased flux through FAT/CD36.
ISSN: 0193-1849
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Faculty of Medicine, Campus Kulak Kortrijk
× corresponding author
# (joint) last author

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