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Title: The effect of plasmin on platelet function
Authors: Blockmans, Daniel Engelbert ×
Deckmyn, Hans
Van den hove, Luc
Vermylen, Jozef #
Issue Date: 1996
Publisher: Carfax publ co
Series Title: Platelets vol:7 issue:3 pages:139-148
Abstract: There is controversy in the literature regarding the effects of plasmin on human platelets, We have studied the effects of plasmin on platelet glycoproteins, aggregation, shape change and secretion and found them to be dependent on experimental conditions: (a) Plasmin's effects on human platelets are only seen in gel-filtered platelets (GFP), presumably because in platelet rich plasma plasmin is bound to alpha 2-antiplasmin; (b) in GFP to which fibrinogen has been added, platelet function remains intact; and (c) in the absence of fibrinogen, the effect of plasmin on GFP depends on whether stirring is performed or not, With stirring, platelets undergo shape change, secretion and aggregation in response to added plasmin. Aggregation is much stronger when CaCl2 1 mM is added, Without stirring, preincubation of GFP with plasmin leads to inhibition of platelet aggregation induced by subsequent platelet stimuli (thrombin, collagen, ristocetin or U46619), We have demonstrated that plasmin is a true platelet activating agent, in the sense that it induces platelet shape change and secretion, Plasmin will induce aggregation when added to stirred GFP. This may be because stirring protects glycoprotein (GP) IIbIIIa bound fibrinogen from being degradated by plasmin, When added to unstirred GFP, GP IIbIIIa bound fibrinogen may be readily accessible to degradation by plasmin, which may then behave like a platelet inhibitor.
ISSN: 0953-7104
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Clinical Infectious and Inflammatory Disorders
Electrical Engineering - miscellaneous
Molecular and Vascular Biology
Chemistry, Campus Kulak Kortrijk
Interdisciplinary Research Facility Life Sciences, Campus Kulak Kortrijk
Faculty of Medicine - miscellaneous
× corresponding author
# (joint) last author

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