Title: Thermodynamics of mn2+-binding to goat alpha-lactalbumin
Authors: Desmet, Johan
Tieghem, E
Van Dael, Herman
Vancauwelaert, F #
Issue Date: 1991
Publisher: Springer verlag
Series Title: European biophysics journal with biophysics letters vol:20 issue:5 pages:263-268
Abstract: By means of reaction calorimetry we measured the apparent enthalpy change, DELTA-H(app), of the binding of Mn2+-ions to goat alpha-lactalbumin as a function of temperature. The observed DELTA-H(app) can be written as the sum of contributions resulting from a conformational and a binding process. In combination with the thermal unfolding curve of goat alpha-lactalbumin, we succeeded in separating the complete set of thermodynamic parameters (DELTA-H, DELTA-G, DELTA-S, DELTA-C(p)) into the binding and conformational contributions. By circular dichroism we showed that NH4+-ions, upon binding to bovine alpha-lactalbumin, induce the same conformational change as do Na+ and K+: the binding constant K(app)NH4+ equals 98 +/- 9 M-1.
ISSN: 0175-7571
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Electrical Engineering - miscellaneous
Faculty of Science, Campus Kulak Kortrijk
# (joint) last author

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