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Title: Delineation of the Salmonella enterica serovar typhimurium HilA regulon through genome-wide location and transcript analysis
Authors: Thijs, Inge ×
De Keersmaecker, Sigrid
Fadda, Abeer
Engelen, Kristof
Zhao, Hui
McClelland, Michael
Marchal, Kathleen
Vanderleyden, Jozef #
Issue Date: Jul-2007
Publisher: American Society for Microbiology (ASM)
Series Title: Journal of Bacteriology vol:189 issue:13 pages:4587-4596
Abstract: The Salmonella enterica serovar Typhimurium HilA protein is the key regulator for the invasion of epithelial cells. By a combination of genome-wide location and transcript analysis, the HilA-dependent regulon has been delineated. Under invasion-inducing conditions, HilA binds to most of the known target genes and a number of new target genes. The sopB, sopE, and sopA genes, encoding effector proteins secreted by the type III secretion system on Salmonella pathogenicity island I (SPI-1), were identified as being both bound by HilA and differentially regulated in an HilA mutant. This suggests a cooperative role for HilA and InvF in the regulation of SPI-1-secreted effectors. Also, siiA, the first gene of SPI-4, is both bound by HilA and differentially regulated in an HilA mutant, thus linking this pathogenicity island to the invasion key regulator. Finally, the interactions of HilA with the SPI-2 secretion system gene ssaH and the flagellar gene flhD imply a repressor function for HilA under invasion-inducing conditions.
URI: 
ISSN: 0021-9193
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Laboratory of Translational Genetics (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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