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Title: Thermodynamic data on the binding of 6 m2+-ions to bovine, goat, and human alpha-lactalbumin
Authors: Desmet, Johan
Haezebrouck, Petra
Vancauwelaert, F #
Issue Date: May-1991
Publisher: Elsevier science inc
Series Title: Journal of inorganic biochemistry vol:42 issue:2 pages:139-145
Abstract: By batch microcalorimetry we titrated the apo-forms of bovine, goat, and human alpha-lactalbumin with Mg2+, Ca2+, Sr2+, Ba2+, Mn2+, and Cd2+ ions at pH 7.5 and 25-degrees-C. The titration curves enabled us to calculate the apparent enthalpy changes and binding constants and thus, also the free energy and the entropy changes of the binding. CD-spectra showed that all cations induce the same conformational change to the native form of the protein. The calorimetric and spectroscopic results, as well as sequence comparisons confirm the hypothesis that all these ions occupy the very same site on the molecule. The thermodynamic parameters, plotted vs the ionic radii, run parallel for the three proteins, which illustrates the earlier proposed "rigid site" model.
URI: 
ISSN: 0162-0134
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Electrical Engineering - miscellaneous
Faculty of Science, Campus Kulak Kortrijk
# (joint) last author

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