Title: N-Acetyl Cysteine Attenuates the Inflammatory Response in Warm Ischemic Pig Lungs
Authors: Geudens, Nele ×
Wuyts, Wim
Rega, Filip
Vanaudenaerde, Bart
Neyrinck, Arne
Verleden, Geert
Lerut, Antoon
Van Raemdonck, Dirk #
Issue Date: 15-May-2008
Publisher: Elsevier
Series Title: The Journal of Surgical Research vol:146 issue:2 pages:177-183
Abstract: BACKGROUND: Lungs donated after cardiac death (DCD) may significantly reduce current organ shortage. However, the warm ischemic period following circulatory arrest may enhance ischemia-reperfusion injury (IRI). We investigated the possible therapeutic effect of N-acetyl cysteine (NAC), a potent anti-oxidative agent on IRI in a porcine ex vivo lung reperfusion model. MATERIALS AND METHODS: NAC (50 mg/kg) was nebulized to pigs (n = 6/group) prior to sacrifice (NAC-DCD). In DCD-NAC, animals received NAC 15 min after death. Control animals did not receive an aerosol (DCD). Interleukin (IL)-1beta, tumor necrosis factor-alpha, IL-8, lactate dehydrogenase activity and thiobarbituric acid reactive substances were measured and cells were counted in broncho-alveolar lavage from the right lung after a 3-h warm plus 1-h cold ischemic interval. RESULTS: There were no differences in cells between groups, however cell death was lower in NAC-DCD (10.3 +/- 1.5%) and DCD-NAC (7.83 +/- 1.8%) compared to DCD (18.0 +/- 3.8%). IL-1beta levels (111.5 +/- 28.8 pg/mL and 92.2 +/- 51.0 pg/mL versus 250.3 +/- 56.6 pg/mL) and lactate dehydrogenase activity (1258.0 +/- 440.9 U/L and 1606.0 +/- 289.0 U/L versus 2848.0 +/- 760.9 U/L) were significantly lower in NAC-DCD and DCD-NAC compared with DCD, respectively. These postischemic inflammatory markers correlated with functional parameters upon reperfusion of the left lung, reported in a previous study. CONCLUSIONS: Administration of NAC prior to or shortly after circulatory arrest results in a marked reduction of inflammation during the warm ischemic phase.
ISSN: 0022-4804
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Thoracic Surgery
Anesthesiology and Algology
Clinical Cardiac Surgery
× corresponding author
# (joint) last author

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