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Title: The early developmental gene Semaphorin 5c contributes to olfactory behavior in adult Drosophila
Authors: Rollmann, Stephanie M ×
Yamamoto, Akihiko
Goossens, Tim
Zwarts, Liesbeth
Callaerts-Vegh, Zsuzsanna
Callaerts, Patrick
Norga, Koenraad
Mackay, Trudy F C
Anholt, Robert R H #
Issue Date: May-2007
Publisher: Genetics Society of America
Series Title: Genetics vol:176 issue:2 pages:947-956
Abstract: Behaviors are complex traits influenced by multiple pleiotropic genes. Understanding the mechanisms that give rise to complex behaviors requires an understanding of how variation in transcriptional regulation shapes nervous system development and how variation in brain structure influences an organism's ability to respond to its environment. To begin to address this problem, we used olfactory behavior in Drosophila melanogaster as a model and showed that a hypomorphic transposon-mediated mutation of the early developmental gene Semaphorin-5c (Sema-5c) results in aberrant behavioral responses to the repellant odorant benzaldehyde. We fine mapped this effect to the Sema-5c locus using deficiency mapping, phenotypic reversion through P-element excision, and transgenic rescue. Morphometric analysis of this Sema-5c allele reveals subtle neuroanatomical changes in the brain with a reduction in the size of the ellipsoid body. High-density oligonucleotide expression microarrays identified 50 probe sets with altered transcriptional regulation in the Sema-5c background and quantitative complementation tests identified epistatic interactions between nine of these coregulated genes and the transposon-disrupted Sema-5c gene. Our results demonstrate how hypomorphic mutation of an early developmental gene results in genomewide transcriptional consequences and alterations in brain structure accompanied by profound impairment of adult behavior.
URI: 
ISSN: 0016-6731
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Behavioral and Developmental Genetics
Associated Laboratories - miscellaneous (-)
Pediatric Hematology & Oncology Section (-)
Laboratory for Biological Psychology
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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