Title: Replication of Legionella pneumophila in biofilms of water distribution pipes
Authors: Declerck, Priscilla ×
Behets, Jonas
Margineanu, Anca
van Hoef, Vincent
De Keersmaecker, Brenda
Ollevier, Frans #
Issue Date: 2009
Publisher: G. Fischer
Series Title: Microbiological Research vol:164 issue:6 pages:593-603
Abstract: Biofilms similar to those present in water distribution pipes of anthropogenic aquatic systems were simulated in a rotating annular reactor using a non-Legionella community consisting of Aeromonas hydrophila, Escherichia coli, Flavobacterium breve and Pseudomonas aeruginosa. The impact of this community and Acanthamoeba castellanii on the replication of Legionella pneumophila was investigated. Despite the presence of 10(7) non-Legionella bacteria, culture and real-time polymerase chain reaction (PCR) results clearly showed that biofilm-associated Legionella bacteria only increased after intracellular replication in A. castellanii. Fluorescent in situ hybridization (FISH) staining of biofilm samples revealed that 48h after addition of amoebae to the reactor, the amoeba population was lysing and replicated Legionella bacteria were released into the bulk water. This study demonstrated that amoebae like A. castellanii can play a crucial role in the increase and spread of L. pneumophila in anthropogenic aquatic systems and thus in the occurrence of Legionnaires' disease.
ISSN: 0944-5013
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Ecology, Evolution and Biodiversity Conservation Section
Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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