Title: The role of hexose transport and phosphorylation in cAMP signalling in the yeast Saccharomyces cerevisiae
Authors: Rolland, Filip
Wanke, Valeria
Cauwenberg, Liesbet
Ma, Pingsheng
Boles, E
Vanoni, M
de Winde, J H
Thevelein, Johan
Winderickx, Joris # ×
Issue Date: Mar-2001
Publisher: Blackwell publishing
Series Title: FEMS Yeast Research vol:1 issue:1 pages:33-45
Abstract: Glucose-induced cAMP signalling in Saccharomyces cerevisiae requires extracellular glucose detection via the Gpr1-Gpa2 G-protein coupled receptor system and intracellular glucose-sensing that depends on glucose uptake and phosphorylation. The glucose uptake requirement can be fulfilled by any glucose carrier including the Gal2 permease or by intracellular hydrolysis of maltose. Hence, the glucose carriers do not seem to play a regulatory role in cAMP signalling. Also the glucose carrier homologues, Snf3 and Rgt2, are not required for glucose-induced cAMP synthesis. Although no further metabolism beyond glucose phosphorylation is required, neither Glu6P nor ATP appears to act as metabolic trigger for cAMP signalling. This indicates that a regulatory function may be associated with the hexose kinases. Consistently, intracellular acidification, another known trigger of cAMP synthesis, can bypass the glucose uptake requirement but not the absence of a functional hexose kinase. This may indicate that intracellular acidification can boost a downstream effect that amplifies the residual signal transmitted via the hexose kinases when glucose uptake is too low.
ISSN: 1567-1356
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Physiology of Plants and Micro-organisms Section - miscellaneous
Molecular Microbiology and Biotechnology Section - miscellaneous (-)
AVL Teacher Training Centre - Biomedical Sciences
× corresponding author
# (joint) last author

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