Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biochemistry & Molecular Biology, Anthracene dipropionic acid, Confocal microscopy, Fluorescent probe, HeLa cells, Silica nanoparticles, Singlet oxygen, MOLECULAR-OXYGEN, PHOTODYNAMIC THERAPY, QUANTUM YIELDS, NANOPARTICLES, GENERATION, STATE, PROBE, LUMINESCENCE, XANTHENE, LIFETIME, Anthracenes, Cytoplasm, Fluorescent Dyes, HeLa Cells, Humans, Nanoparticles, Silicon Dioxide, Singlet Oxygen, Hela Cells, 1103 Clinical Sciences, 3101 Biochemistry and cell biology

Abstract:

We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.