Genomic and Epidemiological Surveillance of Zika Virus in the Amazon Region

Giovanetti, Marta; Faria, Nuno Rodrigues; Lourenço, José; Goes de Jesus, Jaqueline; Xavier, Joilson; Morales Claro, Ingra; Kraemer, Moritz UG; Fonseca, Vagner; Dellicour, Simon; Thézé, Julien; da Silva Salles, Flávia Cristina; Gräf, Tiago; Silveira, Paola Paz; Nascimento, Valdinete Alves Do; Costa de Souza, Victor; Campos de Melo Iani, Felipe; Castilho-Martins, Emerson Augusto; Nogueira Cruz, Laura; Wallau, Gabriel; Fabri, Allison; Levy, Flávia; Quick, Joshua; de Azevedo, Vasco; Santana Aguiar, Renato; de Oliveira, Tulio; Bôtto de Menezes, Camila; da Costa Castilho, Marcia; Terra, Tirza Matos; Souza da Silva, Marineide; Bispo de Filippis, Ana Maria; Luiz de Abreu, André; Oliveira, Wanderson Kleber; Croda, Julio; Campelo de Albuquerque, Carlos F; Nunes, Marcio RT; Cerdeira Sabino, Ester; Loman, Nicholas; Gomes Naveca, Felipe; Pybus, Oliver G; Alcantara, Luiz Carlos

doi:10.1016/j.celrep.2020.01.085

Genomic and Epidemiological Surveillance of Zika Virus in the Amazon Region

Author:

Giovanetti, Marta

Faria, Nuno Rodrigues ; Lourenço, José ; Goes de Jesus, Jaqueline ; Xavier, Joilson ; Morales Claro, Ingra ; Kraemer, Moritz UG ; Fonseca, Vagner ; Dellicour, Simon ; Thézé, Julien ; da Silva Salles, Flávia Cristina ; Gräf, Tiago ; Silveira, Paola Paz ; Nascimento, Valdinete Alves Do ; Costa de Souza, Victor ; Campos de Melo Iani, Felipe ; Castilho-Martins, Emerson Augusto ; Nogueira Cruz, Laura ; Wallau, Gabriel ; Fabri, Allison ; Levy, Flávia ; Quick, Joshua ; de Azevedo, Vasco ; Santana Aguiar, Renato ; de Oliveira, Tulio ; Bôtto de Menezes, Camila ; da Costa Castilho, Marcia ; Terra, Tirza Matos ; Souza da Silva, Marineide ; Bispo de Filippis, Ana Maria ; Luiz de Abreu, André ; Oliveira, Wanderson Kleber ; Croda, Julio ; Campelo de Albuquerque, Carlos F ; Nunes, Marcio RT ; Cerdeira Sabino, Ester ; Loman, Nicholas ; Gomes Naveca, Felipe ; Pybus, Oliver G ; Alcantara, Luiz Carlos

Keywords:

Science & Technology, Life Sciences & Biomedicine, Cell Biology, PHYLOGENETIC ANALYSIS, TRANSMISSION, OUTBREAK, SPREAD, BRAZIL, OROPOUCHE, REVEALS, MODELS, ANGOLA, FEVER, Brazil, Epidemiological Monitoring, Female, Genomics, Humans, Male, Zika Virus, Zika Virus Infection, 0601 Biochemistry and Cell Biology, 1116 Medical Physiology, 31 Biological sciences

Abstract:

Zika virus (ZIKV) has caused an explosive epidemic linked to severe clinical outcomes in the Americas. As of June 2018, 4,929 ZIKV suspected infections and 46 congenital syndrome cases had been reported in Manaus, Amazonas, Brazil. Although Manaus is a key demographic hub in the Amazon region, little is known about the ZIKV epidemic there, in terms of both transmission and viral genetic diversity. Using portable virus genome sequencing, we generated 59 ZIKV genomes in Manaus. Phylogenetic analyses indicated multiple introductions of ZIKV from northeastern Brazil to Manaus. Spatial genomic analysis of virus movement among six areas in Manaus suggested that populous northern neighborhoods acted as sources of virus transmission to other neighborhoods. Our study revealed how the ZIKV epidemic was ignited and maintained within the largest urban metropolis in the Amazon. These results might contribute to improving the public health response to outbreaks in Brazil.