Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Cell Biology, Biochemistry & Molecular Biology, Metastasis, Metabolism, In vivo metabolism, C-13 tracer analysis, Metabolomics, Mouse infusions, TCA CYCLE, MITOCHONDRIAL METABOLISM, PYRUVATE-CARBOXYLASE, CANCER METABOLISM, OXIDATION, BIOSYNTHESIS, 13C tracer analysis, Animals, Carbon Isotopes, Cell Line, Tumor, Disease Models, Animal, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Neoplasm Metastasis, Neoplasms, Rats, Rats, Sprague-Dawley, 0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology, Developmental Biology, 3101 Biochemistry and cell biology, 3404 Medicinal and biomolecular chemistry

Abstract:

Metastasis formation is the leading cause of death in cancer patients. It has recently emerged that cancer cells adapt their metabolism to successfully transition through the metastatic cascade. Consequently, measuring and analyzing the in vivo metabolism of metastases has the potential to reveal novel treatment strategies to prevent metastasis formation. Here, we describe two different metastasis mouse models and how their metabolism can be analyzed with metabolomics and 13C tracer analysis.