Title: Nucleotide-sequence analysis of an 8887-bp region of the left arm of yeast chromosome xiv, encompassing the centromere sequence
Authors: Verhasselt, P ×
Aert, Rita
Voet, Maria-Helena
Volckaert, Guido #
Issue Date: Jul-1994
Publisher: John wiley & sons ltd
Series Title: Yeast vol:10 issue:7 pages:945-951
Abstract: The nucleotide sequencing of 8887 bp of the left arm of chromosome XIV is described. The sequence includes the centromeric region. Both strands were sequenced with an average redundancy of 5.09 per base pair. The overall G+C content is 37.3% (39.2% for putative coding regions versus 32.5% for non-coding regions). Six open reading frames (ORFs) greater than 100 amino acids were detected, all of which are completely confined to the 8.9 kbp region. Codon frequencies of the six ORFs agree with codon usage in Saccharomyces cerevisiae and all show the characteristics of low-level expressed genes. Comparison of the translated sequences with protein sequences in data bases suggests the presence of two ORFs (N2014 and N2007) encoding ribosomal proteins, the latter of which is the previously sequenced MRP7 gene. Another ORF (N2012) could encode a membrane-associated protein since it contains secretory signal sequence and two presumed transmembrane helices. This protein might be involved in mitochondrial energy transfer. ORF N2016 is immediately adjacent to the centromere, suggesting that it corresponds to the SPO1 gene, which is very tightly linked to the centromere at the left arm side of chromosome XIV (Mortimer et al., 1989). The sequence has been deposited in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the Accession Number X77114.
ISSN: 0749-503X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
Division of Gene Technology (-)
× corresponding author
# (joint) last author

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