Title: Rocket-powered high-performance liquid chromatographic analysis of plant ascorbate and glutathione
Authors: Davey, Mark ×
Dekempeneer, E
Keulemans, Johan #
Issue Date: May-2003
Publisher: Academic press inc elsevier science
Series Title: Analytical biochemistry vol:316 issue:1 pages:74-81
Abstract: We describe a robust procedure for the extraction and high-performance liquid chromatographic analysis Of L-ascorbate (vitamin C), glutathione (gamma-glutamyl cysteinylglycine), and their respective oxidized forms from various plant tissues. Parameters such as the choice of extraction buffer, tissue disruption technique, sample stability, and separation conditions have all been optimized. In particular we found that the inclusion of the reducing agent dithiothreitol as a "stabilizer" in extracts with high phenolic content actually promoted oxidation of these antioxidants. Further, by using commercially available short "Rocket" HPLC columns in combination with high mobile-phase flow rates, analysis times were reduced to only 6 min, making the method suitable for the high-resolution screening of large numbers of samples. (C) 2003 Elsevier Science (USA). All rights reserved.
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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