The effect of bromocryptine on milk production was investigated in 32 crossbred ewes (Flemish Milksheep X Suffolk) with one sucking lamb, during lactation and during drying off. After weaning the lambs, all ewes were hand milked twice daily for 3 days. From day 4 onwards, the ewes were divided into four groups with equal mean daily milk production. Ewes of groups A and B were hand milked twice daily during the following 9 days. Ewes of groups C and D were dried off by reducing the milking frequency. They were milked on days 5, 8 and 12. In groups B and D, bromocryptin (1 ml of 1 g/l ethanol 50%) was injected intramuscularly twice daily while control ewes (groups A and C) received 1 ml ethanol 50%. Blood samples were taken by venipuncture prior to each injection and were assayed for prolactin, growth hormone, thyrotropin and cortisol.