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Title: Transient gene-expression in electroporated banana (musa spp, cv bluggoe, abb group) protoplasts isolated from regenerable embryogenetic cell-suspensions
Authors: Sagi, Laszlo ×
Remy, Serge
Panis, Bart
Swennen, Rony
Volckaert, Guido #
Issue Date: Feb-1994
Publisher: Springer verlag
Series Title: Plant cell reports vol:13 issue:5 pages:262-266
Abstract: Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv.'Bluggoe') protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol treatment and its duration before electroporation, use of a heat shock, and chimaeric gene constructs. The maximum frequency of DNA introduction as detected by an in situ assay for transient expression of the uidA gene, amounted to 1.8% of total protoplasts. Since plants have recently been regenerated from banana protoplasts at a high frequency, the present results may contribute to the production of transgenic banana.
ISSN: 0721-7714
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
Division of Gene Technology (-)
× corresponding author
# (joint) last author

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