Title: Cryopreservation of embryogenic tissues of Pinus nigra Arn. by a slow freezing method
Authors: Salaj, Terezia ×
Panis, Bart
Swennen, Rony
Salaj, Jan #
Issue Date: Mar-2007
Publisher: Cryo letters
Series Title: Cryoletters vol:28 issue:2 pages:69-76
Abstract: Six different embryogenic cell lines of Pinus nigra Am. have been cryopreserved in liquid nitrogen using cryoprotection with sucrose (18%) and DMSO (7.5%). Post-thaw growth and tissue proliferation have been observed in five cell lines. The survival levels after storage in liquid nitrogen reached values between 62.5 and 100%. Growth of recovered embryogenic cells as well as somatic embryos is similar to the non-frozen tissues maintained in long-term culture. Somatic embryo maturation and plantlet regeneration occurred in all selected cell lines.
ISSN: 0143-2044
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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