Title: Protoplast-to-plant regeneration in cotton (gossypium-hirsutum l-cv coker 312) using feeder layers
Authors: Peeters, Christine ×
Willems, K
Swennen, Rony #
Issue Date: Jan-1994
Publisher: Springer verlag
Series Title: Plant cell reports vol:13 issue:3-4 pages:208-211
Abstract: We report the regeneration of protoplasts isolated from two embryogenic cell lines of Gossypium hirsutum L. cv. Coker 312 initiated from hypocotyl-derived callus. Protoplasts plated on cellulose nitrate filters and placed over feeder layers formed embryogenic callus from which plants were regenerated. Plating efficiency up to 12.8% depended upon the cell line. Addition of phytohormones to the protoplast medium had no stimulating effect on plating efficiency. The influence of feeder cells and conditioned medium on plating efficiency was significantly different for the two cell lines.
ISSN: 0721-7714
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Teaching Support Unit, Faculty of Bioscience Engineering
Division of Crop Biotechnics
× corresponding author
# (joint) last author

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