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Title: Plant-regeneration through direct somatic embryogenesis from protoplasts of banana (Musa spp)
Authors: Panis, Bart ×
Van Wauwe, A
Swennen, Rony #
Issue Date: May-1993
Publisher: Springer verlag
Series Title: Plant cell reports vol:12 issue:7-8 pages:403-407
Abstract: We report the isolation and regeneration of protoplasts from an embryogenic banana (Musa spp.) cell suspension culture initiated from in vitro proliferating meristems. A high yielding isolation method (up to 6x10(7) protoplasts.ml-1 packed cells) is discussed. Optimal regeneration, with more than 50 % of the protoplasts showing initial cell division, occurred when high inoculation densities (10(6) protoplasts.ml-1) or nurse cultures were applied. Under these conditions, the frequency of microcolony formation was 20-40 %. These microcolonies developed directly, without an intervening callus phase, into somatic embryos which later germinated and formed plantlets.
ISSN: 0721-7714
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
× corresponding author
# (joint) last author

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