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Title: Efficient expression of bovine alpha-lactalbumin in saccharomyces-cerevisiae
Authors: Viaene, A ×
Volckaert, Guido
Joniau, Marcel
Debaetselier, A
Vancauwelaert, F #
Issue Date: Dec-1991
Publisher: Springer verlag
Series Title: European Journal of Biochemistry vol:202 issue:2 pages:471-477
Abstract: A synthetic gene encoding the mature bovine alpha-lactalbumin fused to the preproregion of the yeast alpha-mating factor has been expressed and secreted at high level in Saccharomyces cerevisiae under the control of the alpha-mating promotor. Growth conditions were found to be critical for the expression: recombinant alpha-lactalbumin could only be detected in the medium provided the culture was grown at neutral pH. The secreted bovine alpha-lactalbumin is enzymatically active and identical to the whey protein, as confirmed by SDS/PAGE, IEF, ultraviolet and CD spectral analysis, and amino-terminal sequence determination.
ISSN: 0014-2956
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Faculty of Medicine, Campus Kulak Kortrijk
× corresponding author
# (joint) last author

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