ITEM METADATA RECORD
Title: Acute pretranslational regulation of type III iodothyronine deiodinase by growth hormone and dexamethasone in chicken embryos
Authors: Van der Geyten, Serge ×
Buys, Nadine
Sanders, JP
Decuypere, Eddy
Visser, TJ
Kühn, Eduard
Darras, Veerle #
Issue Date: Jan-1999
Publisher: Elsevier sci ireland ltd
Series Title: Molecular and cellular endocrinology vol:147 issue:1-2 pages:49-56
Abstract: Both growth hormone (GH) and glucocorticoids are regulators of thyroid hormone metabolism in vertebrates. Studies on chicken embryos demonstrated that intravenous (iv) injection of chicken GH or glucocorticoids results in increased plasma 3,3',5-triiodothyronine (T-3) concentrations, and this through a reduction of hepatic type III iodothyronine deiodinase (D3) activity. The recent cloning of chicken type I iodothyronine deiodinase (D1) and D3 offers the tools to investigate at what level (pre- or posttranslational) this downregulation of D3 occurs. Eighteen day old chicken embryos were injected with either 0.9% NaCl (control), 50 mu g dexamethasone (DEX), or 20 mu g cGH per animal. Plasma and tissue samples were taken 5, 10, 30, 60, 120, and 240 min post-injection. Plasma T-3 and thyroxine (T-4) were determined as well as in vitro hepatic DI and D3 activities. Hepatic D1 and D3 mRNA levels were measured by both Northern analysis and competitive reverse transcription polymerase chain reaction (RT-PCR). Injection of GH or DEX resulted in a significant increase in plasma T-3 when compared to controls within 30 min post-injection. This increase remained until the end of the experiment in the DEX-treated group, but not in the GH group. GH administration had no influence on plasma T-4 levels, whereas DEX significantly reduced plasma T-4 from 30 min onwards. Hepatic D1 activity and D1 mRNA levels showed no changes. Hepatic D3 activity, however, decreased within 10 min after DEX administration and somewhat slower after GH administration (within 30 min). Hepatic D3 activity remained low for the remainder of the experiment in the DEX-treated group, whereas D3 activity gradually returned to control levels in the GH group. This change in hepatic D3 activity was paralleled by the changes in hepatic D3 mRNA levels (r = 0.88, P = 0.0001) as confirmed by both Northern analysis and competitive RT-PCR. In conclusion, these results demonstrate that in embryonic chicken GH and DEX acutely increase plasma T-3 levels by decreasing hepatic D3 activity, a decrease that seems to be regulated predominantly at the pretranslational level. These results are also an indication for the short half life (t(1/2)) of the D3 enzyme. The time lag between the effect of GH and DEX on hepatic D3 mRNA may be due to differences in the mechanism of action between both hormones, a subject that needs further investigation. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
URI: 
ISSN: 0303-7207
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Division of Gene Technology (-)
Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Van der Geyten et al._1999_Molecular and Cellular Endocrinology_vol147_p49-56.pdf Published 340KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members

 




All items in Lirias are protected by copyright, with all rights reserved.

© Web of science