Immunocytochemical demonstration of chicken hypophyseal thyrotropes and development of a radioimmunological indicator for chicken tsh using monoclonal and polyclonal homologous antibodies in a subtractive strategy
General and Comparative Endocrinology vol:92 issue:2 pages:189-200
The production and characterization of monoclonal antibodies (mAbs) toward the chicken pituitary glycoproteins are described. MAbs were first subdivided on an immunocytochemical (ICC) basis: one category of mAbs reacts with both thyrotropic and gonadotropic cells whereas another stains exclusively gonadotropic cells. The hypothesis that the former are anti-glycoprotein and that the latter are anti-βLH and/or anti-βFSH mAbs was further elaborated in a homologous tracer binding assay. The putative anti-β gonadotropin mAbs exclusively recognize iodinated USDA-cLH-I-1, whereas the supposed anti- mAbs react with both USDA-cLH-I-1 and USDA-cFSH-I-1. The ICC localization of pituitary thyrotropes has been achieved in a double-staining protocol using an anti-LH mAb to subtract specifically the gonadotropic cells from the complete -subunit-containing cell population. The result is a strictly cephalic cell population that is densely granulated in hyperthyroid and hardly detectable in hypothyroid birds. A similar subtractive approach was used to develop a thyroid-stimulating hormone (TSH)-indicator in RIA. The total titer of -containing molecules is first quantified with an anti- mAb. The contribution of both LH and FSH to this titer is then measured by homologous USDA-RIA and subtracted from the total -immunoreactivity to give an indication of the TSH level of the sample. The direct effect of thyroid-releasing hormone on pituitary TSH release in vitro in juvenile chicks and the effect of hypo- and hyperthyroidism on circulating TSH levels in vivo in juvenile broilers have been demonstrated.