Title: Characterization of mature rat oligodendrocytes: a proteomic approach
Authors: Dumont, Debora ×
Noben, Jean-Paul
Moreels, Marjan
Vanderlocht, Joris
Hellings, Niels
Vandenabeele, Frank
Lambrichts, Ivo
Stinissen, Piet
Robben, Johan #
Issue Date: Jul-2007
Publisher: Blackwell publishing
Series Title: Journal of Neurochemistry vol:102 issue:2 pages:562-576
Abstract: Oligodendrocytes are glial cells responsible for the synthesis and maintenance of myelin in the central nervous system (CNS). Oligodendrocytes are vulnerable to damage occurring in a variety of neurological diseases. Understanding oligodendrocyte biology is crucial for the dissemination of de- and remyelination mechanisms. The goal of the present study is the construction of a protein database of mature rat oligodendrocytes. Post-mitotic oligodendrocytes were isolated from mature Wistar rats and subjected to immunocytochemistry. Proteins were extracted and analyzed by means of two-dimensional gel electrophoresis and two-dimensional liquid chromatography, both coupled to mass spectrometry. The combination of the gel-based and gel-free approach resulted in confident identification of a total of 200 proteins. A minority of proteins were identified in both proteomic strategies. The identified proteins represent a variety of functional groups, including novel oligodendrocyte proteins. The results of this study emphasize the power of the applied proteomic strategy to study known or to reveal new proteins and to investigate their regulation in oligodendrocytes in different disease models.
ISSN: 0022-3042
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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