Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biochemistry & Molecular Biology, Cell Biology, CD8(+) T cells, Immunometabolism, C-13 tracer analysis, Nutrient microenvironment, Custom media formulations, IMMUNOMETABOLISM, GUIDE, 13C tracer analysis, CD8+ T cells, Animals, CD8-Positive T-Lymphocytes, Carbon Isotopes, Cell Differentiation, Cells, Cultured, Culture Media, Lymphocyte Activation, Mass Spectrometry, Metabolomics, Mice, Mice, Inbred C57BL, Nutrients, Primary Cell Culture, 0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology, Developmental Biology, 3101 Biochemistry and cell biology, 3404 Medicinal and biomolecular chemistry

Abstract:

Immune cell function is tightly regulated by cellular metabolism, which in turn is strongly linked to the nutrient availability in the microenvironment surrounding the cells. This link is critical for effector CD8+ T cells which, after activation, must migrate from nutrient-rich environments into nutrient-scarce regions such as the tumor microenvironment. Assessing how nutrient availability modulates the metabolism of effector CD8+ T cells is thus key for understanding how harsh environments may impair their proliferation and effector function. Here, we describe an approach to systematically study the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells, based on performing stable 13C isotope labeling measurements on in vitro-differentiated murine effector CD8+ T cells.