Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biochemistry & Molecular Biology, Cell Biology, ATP synthesis, Oxidative phosphorylation, beta-oxidation, Mitochondria, Bioenergetics, Oxidative phosphorylation (OXPHOS), β-oxidation, Adenosine Triphosphate, Cell Line, Electron Transport Complex I, Electron Transport Complex II, High-Throughput Screening Assays, Humans, Metabolomics, Mitochondrial Membranes, C14/17/110#54271219, 0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology, Developmental Biology, 3101 Biochemistry and cell biology, 3404 Medicinal and biomolecular chemistry

Abstract:

Here, we offer you a high-throughput assay to measure the ATP synthesis capacity in cells or isolated mitochondria. More specifically, the assay is linked to the mitochondrial' electron transport chain components of your interest being either through complex I (with or without a linkage to pyruvate dehydrogenase activity), through complex II, or through the electron transport flavoprotein and complex I (β-oxidation of fatty acids).