Title: A standardized approach for accurate quantification of murein hydrolase activity in high-throughput assays
Authors: Briers, Yves ×
Lavigne, Rob
Volckaert, Guido
Hertveldt, Kirsten #
Issue Date: Apr-2007
Publisher: Elsevier science bv
Series Title: Journal of biochemical and biophysical methods vol:70 issue:3 pages:531-533
Abstract: Current spectrophotometers measure murein hydrolase activity simultaneously under many conditions and in small intervals. A correct interpretation of these large data sets requires clear and standardized criteria. Furthermore, there is a need for a uniform unit definition to express enzymatic activity, because application of variable definitions seriously hampered comparison between different studies. The method presented here is based on maximizing R-2-values of incremental data sets. Combined with an appropriate unit definition, it provides a statistically sound background and warrants reproducible and reliable results. Activity calculations are further simplified by an online available Excel spreadsheet. This method is especially suited for experiments where individual curves differ extensively from each other (e.g. low versus high activity conditions) and can be expanded to other similar high-throughput bioassays. (c) 2006 Elsevier B.V. All rights reserved.
ISSN: 0165-022X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
× corresponding author
# (joint) last author

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