Title: Flow cytometry as a quantitative and sensitive method to evaluate low dose radiation induced apoptosis in vitro in human peripheral blood lymphocytes
Authors: Hertveldt, Kirsten ×
Philippe, J
Thierens, H
Cornelissen, M
Vral, A
Deridder, L #
Issue Date: Apr-1997
Publisher: Taylor & Francis
Series Title: International Journal of Radiation Biology vol:71 issue:4 pages:429-433
Abstract: Human peripheral blood lymphocytes, irradiated In vitro, die by an apoptotic process. The number of apoptotic cells after in vitro gamma-irradiation (0, 0.1, 0.2, 1, 2 and 5 Gy) was measured by flow cytometry using Annexin V and DiOC(6) (a cationic dye) after 24 and 48h incubation. The mean dose-response curves for apoptosis of six healthy volunteers obtained with both methods were steep below 1Gy and flatter at higher doses. A slightly higher number of apoptotic cells was observed with DiOC(6), compared to Annexin V. This can be assigned to a minor DiOC(6)-int/PI- population. Forty-eight hour cultures contained higher numbers of apoptotic cells compared with 24h cultures. For both culture times, DiOC(6) and Annexin V detected a statistically significant difference between a control sample and a 0.1 Gy irradiated one, illustrating the high sensitivity of the methods.
ISSN: 0955-3002
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
× corresponding author
# (joint) last author

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