Title: Antipeptide antibody against bovine IGF-BP-2: Application to the detection of bovine somatotropin-treated cows
Authors: Scippo, ML ×
Degand, G
Duyckaerts, A
Michel, A
Joris, B
Delahaut, P
Decuypere, Eddy
MaghuinRogister, G #
Issue Date: Mar-1996
Publisher: Carfax publ co
Series Title: Food and agricultural immunology vol:8 issue:1 pages:31-40
Abstract: The aim of this study was to develop a method to identify cows illegally treated with bovine somatotropin (BST). Using the Western ligand blotting technique, BST treatment was shown to cause a dramatic decrease of IGF-BP-2 in the serum of treated cows. Measurement of the insulin-like growth factor-binding protein-2 (IGF-BP-2) concentration in cow plasma could thus be a good way to identify, BST-treated animals. In order to develop an immunoassay specific for bovine IGF-BP-2 polyclonal antibodies against synthetic peptides of bovine IGF-BP-2 were produced Three peptides were chosen in the [150-190] region of the molecule, which display no identity with other IGF-BPs in serum: [154-165], [171-184] and [163-176]. Two antisera were obtained which reacted specifically with the entire bovine IGF-BP-2 molecule, both of which came from rabbits immunized with the [163-176] peptide.
ISSN: 0954-0105
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Livestock-Nutrition-Quality (-)
× corresponding author
# (joint) last author

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