Antipeptide antibody against bovine IGF-BP-2: Application to the detection of bovine somatotropin-treated cows
Scippo, ML × Degand, G Duyckaerts, A Michel, A Joris, B Delahaut, P Decuypere, Eddy MaghuinRogister, G #
Carfax publ co
Food and agricultural immunology vol:8 issue:1 pages:31-40
The aim of this study was to develop a method to identify cows illegally treated with bovine somatotropin (BST). Using the Western ligand blotting technique, BST treatment was shown to cause a dramatic decrease of IGF-BP-2 in the serum of treated cows. Measurement of the insulin-like growth factor-binding protein-2 (IGF-BP-2) concentration in cow plasma could thus be a good way to identify, BST-treated animals. In order to develop an immunoassay specific for bovine IGF-BP-2 polyclonal antibodies against synthetic peptides of bovine IGF-BP-2 were produced Three peptides were chosen in the [150-190] region of the molecule, which display no identity with other IGF-BPs in serum: [154-165], [171-184] and [163-176]. Two antisera were obtained which reacted specifically with the entire bovine IGF-BP-2 molecule, both of which came from rabbits immunized with the [163-176] peptide.