Two experiments were completed to observe the combined effects of feed withdrawal and the catching and transport process on stress and energy metabolism. During one experiment 192 male broilers (46 d of age) were used, and in the other we used 240 male broilers (49 d of age). The experiments consisted of 2 interventions: feed intervention and transport intervention. The feed intervention took 10 h, in which broilers had full access to feed or feed was withdrawn and, thereafter, had a transport intervention that took 3 h, in which broilers were caught, crated, loaded, transported, and then had to wait in the crates for 1 h or remained in the pens. After the transport intervention, blood samples were taken to determine plasma corticosterone, triiodothyronine, thyroxine, glucose, lactate, uric acid, nonesterified fatty acid, and triglyceride concentrations. Changes in BW were also assessed.