Title: Characterization of the bacteriophage Phi KMV DNA ligase
Authors: Lavigne, Rob ×
Roucourt, Bart
Hertveldt, Kirsten
Volckaert, Guido #
Issue Date: Oct-2005
Publisher: Bentham science publ ltd
Series Title: Protein and peptide letters vol:12 issue:7 pages:645-648
Abstract: Gene 17 product (gp17) of the Pseudomonas aeruginosa-infecting bacteriophage phi KMV shows in silico similarity to T7 DNA ligase. In a semi-quantitative activity assay, it is shown that gp17 is a functional, ATP-dependent DNA ligase, in spite of some structural differences related to DNA-binding properties). Enzymatic activity of His(6)-based purified expression product was optimised (4 degrees C at 24h for sticky end double-stranded DNA fragments) and estimated at 0.5 Weiss U/mu g.
ISSN: 0929-8665
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Laboratory of Glycobiology and Developmental Genetics (-)
× corresponding author
# (joint) last author

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