Title: Restrictions of the efficiency of haploid plant-production in apple cultivar idared, through parthenogenesis in-situ
Authors: De Witte, K
Keulemans, Johan #
Issue Date: 1994
Publisher: Kluwer academic publ
Series Title: Euphytica vol:77 issue:1-2 pages:141-146
Abstract: For haploid plant production in Idared apple, parthenogenesis in situ was induced by irradiated pollen. The most important restrictions were seed set, embryo germination and green plant recovery from germinated embryos. The efficiency of green plant production was strongly influenced by irradiation dose, picking time and quality of the irradiated pollen. 250 Gy pollen was more efficient for green plant production than 500 Gy pollen. Using 250 Gy pollen with reduced germination capacity resulted in a five-fold lower green plant yield. Delaying picking of the fruits (140 instead of 70 days after pollination) had a significant positive effect. Up to 20 plants per 1000 pollinated flowers, showing no expression of a marker gene present in the pollen parent, were obtained in some treatments.
ISSN: 0014-2336
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
# (joint) last author

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